Developments in cell biology for quantitative immunoelectron microscopy based on thin sections: a review
نویسندگان
چکیده
منابع مشابه
Cell culture on thin tissue sections commonly prepared for histopathology.
Thin tissue sections commonly prepared on a glass slide for histopathology retain many in vivo biochemical attributes related not only to structure but also function. We hypothesized that such tissue sections might serve as novel cell culture substrata that would reflect tissue conditions in vivo. Here we report the applicability of tissue section substrata to tissue reconstruction and serum-fr...
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Complexes of protein-A with 5 and 16 nm colloidal gold particles (PA/Au5 and PA/Au16) are presented as sensitive and clean immunoprobes for ultrathin frozen sections of slightly fixed tissue. The probes are suitable for indirect labeling and offer the opportunity to mark multiple sites. The best procedure for double labeling was to use the smaller probe first, i.e., antibody 1 - PA/Au5 - antibo...
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ژورنال
عنوان ژورنال: Histochemistry and Cell Biology
سال: 2008
ISSN: 0948-6143,1432-119X
DOI: 10.1007/s00418-008-0451-6